Download the final program and book of abstracts
Sunday, Sept 3rd, 2017
16:00-17:45 – Participant registration and accommodation check-in
17:45-18:00 – Welcome by Organizing Committee
18:00-19:00 – OPENING LECTURE (Chair: A. Mattevi):
Werner Kuhlbrandt (MPI Frankfurt)
Membrane protein complexes and the resolution revolution in cryoEM
19:00-20:30 – Welcome Party
Monday, Sept 4th, 2017
“SAMPLES AND INSTRUMENTS” (Chair: B. Vallone)
09:00-09:40 – Tillmann Pape (Imperial, UK)
THEORY: Introduction to cryo-electron microscopy: from biological sample to 3D structure – the beauty of cryo-EM
09:40-10:25 – Paolo Swuec (University of Milano, IT)
REAL LIFE: Overview of Sample Preparation for Single-particle EM
10:25-10:55 – Frederic Leroux (Leica Microsystems)
Frozen in milliseconds, but what’s next?
10:55-11:20 – Coffee Break
11:20-12:00 – Marta Carroni (SciLife Lab, SE)
THEORY: Electron microscope architecture and image formation
12:00-12:30 – Max Maletta (FEI)
Single particle screening and data acquisition made easy
12:30-14:00 – Lunch
“THE SINGLE-PARTICLE EXPERIMENT” (Chair: S. Tarantino)
14:00-14:40- Fabrizio Martino (CIB Madrid, ES)
REAL LIFE: Theory to Practice: Grid preparation
14:40-15:30 – Arjen J. Jakobi (EMBL Heidelberg, DE)
THEORY: Fundamentals of cryo-EM image processing
15:30-16:00 – Andy Jarwood (JEOL)
Introduction to a New Cryo-TEM generation and associated techniques
16:00-16:30 – Coffee Break
16:30-17:10 – Fabrizio Martino (CIB Madrid, ES)
REAL LIFE: Low cost cryo-EM… the Madrid example
16:30-17:10 – Marta Carroni (SciLife Lab, SE)
REAL LIFE: Different mechanisms but conserved functions: AAA+
protein activity control studied by cryo-EM at various resolutions
17:10-18:00 – Discussion, Q&A
Tuesday, Sept 5th, 2017
“BEYOND SINGLE PARTICLE” (Chair: M. Gemmi)
09:00-09:40 – Christoph A. Diebolder (NeCEN, NL)
THEORY AND REAL LIFE: Cryo electron tomography part I: Concepts
and practical considerations for sample preparation and data collection
09:40-10:20 – Mauro Gemmi (IIT Pisa, IT)
THEORY: Electron Diffraction Tomography of Protein Nanocrystals
10:20-10:50 – Sacha De Carlo (DECTRIS)
Detector technologies for high-resolution TEMs
10:50-11:20 – Coffee Break
11:20-12:05 – Selected young speaker:
Jérôme Bürgi (EMBL Hamburg)
Towards the structural discovery of the peroxisomal translocation of
functional matrix proteins
12:05-12:30 – Selected young speaker:
Digant Nayak (NUS Singapore)
Structural basis for the indispensable role of a unique zinc finger motif
in LNX2 ubiquitination
12:30-14:00 – Lunch
“SOFTWARE PACKAGES AND DATA PROCESSING” (Chair: F. Forneris)
14:00-14:40 – B. Tom Burnley (CCP-EM, STFC, UK)
THEORY: Atomic model building and refinement in CCP-EM
14:40-15:20 – Christoph A. Diebolder (NeCEN, NL)
THEORY: Cryo electron tomography part II: Software packages for data
acquisition, reconstruction and subvolume averaging
15:20-16:00 – Pablo Conesa (I2PC Madrid, ES)
THEORY & REAL LIFE: A software framework toward integration,
reproducibility and validation in 3D electron microscopy
16:00-16:30 – Coffee Break
16:30-17:10 – Arjen J. Jakobi (EMBL Heidelberg, DE)
THEORY & REAL LIFE: Building, refining and validating atomic models
in cryo-EM density maps
17:10-17:40 – Fabrizio Martino (CIB Madrid, ES)
REAL LIFE: Processing toolbox… one problem, many solutions
19:00-22:00 – Social Dinner
Wednedsay, Sept 6th, 2017
“BIOLOGY WITH CRYO-EM” (Chair:G. Zanotti)
09:00-09:40 – Filippo Mancia (Columbia University, US)
The Molecular Mechanisms Underlying Cellular Uptake of Vitamin A
09:40-10:20 – Alessandro Vannini (ICR London, UK)
Structural basis of RNA Polymerase III transcription initiation
10:20-11:00 – Arjen J. Jakobi (EMBL Heidelberg, DE)
Structural basis of selective autophagy receptor assemblies
11:00-11:30 – Coffee Break
11:30-11:55 – Selected young speaker:
Andrew Hedger (University of Queensland)
Introduction to protein filament characterisation
11:55-12:20 – Selected young speaker:
Ilaria Peschiera (CNB Madrid)
Deciphering the oligomerization role of the bacterial lipid rafts
scaffold protein by Cryo-EM
12:30-14:00 – Lunch
“CRYO-EM FACILITIES” (Chair: M. Bolognesi)
14:00-14:25 – Martin Walsh (Diamond eBIC, UK)
Integrating cryo-EM at Diamond…
Challenges, benefits and opportunities
14:25-14:50 – Guy Schoehn (IBS Grenoble, FR)
Current and future state of the EPN cryoEM facility
14:50-15:15 – Marta Carroni (SciLife La, SE)
A Swedish facility for cryo-EM
15:15-15:40 – Paolo Swuec (University of Milano, IT)
Introducing the Cryo-Electron Microscopy Lab in Milan (CEMiL)
15:40-16:05 – Ludo Renault (NeCEN, NL)
Operations at NeCEN
16:05-16:30 – Coffee Break
16:30-18:30 – Social Program
18:30-19:30 – CLOSING LECTURE (Chair: F. Mancia):
Wayne Hendrickson (Columbia University, USA)
Electron Microscopy and X-ray Crystallography of
Ion Channels that Control Muscle Excitation
19:30-20:00 – Concluding Remarks